transforming growth factor beta-1-induced transcript 1 protein (hydrogen peroxide-inducible clone 5 protein, Hic-5, androgen receptor-associated protein of 55 kD, TGFB1I1, ARA55)

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Function

molecular adapter coordinating multiple protein-protein interactions at focal adhesion complex & in nucleus
links various intracellular signaling modules to plasma membrane receptors
regulates the Wnt & TGFB signaling pathways
may regulate SLC6A3 & SLC6A4 targeting to plasma membrane, thus regulating their activity
in nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid receptor, androgen receptor, mineralocorticoid receptor & progesterone receptor transcriptional activity
role in processes of cell growth, proliferation, migration, differentiation & senescence
zinc-dependent DNA-binding activity
interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 & VCL
forms a complex with GIT1 & ARHGEF7
interacts with androgen receptor (ligand-dependent)
interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2, PTK2B, SLC6A3, SLC6A4, SMAD3, SRC & talin
phosphorylated by gonadotropin-releasing hormone-activated SRC

homooligomer
contains 4 LIM zinc-binding domains
LIM zinc-binding domains mediate glucocorticoid receptor coactivation & interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 & SMAD3
LIM zinc-binding 2 & LIM zinc-binding 3 domains mediate targeting to focal adhesions & actin stress fibers
LIM zinc-binding 3 & LIM zinc-binding 4 domains mediate interaction with TRAF4 & MAPK15.
LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization & targeting to the nuclear matrix
LIM zinc-binding 3 domain mediates interaction with PTPN12
LD motif (leucine & aspartate-rich motif) 3 mediates interaction with GIT1 & functions as a nuclear export signal
belongs to the paxillin family