vinculin; metavinculin (VCL)

Function

• role in cell adhesion
• may play roles in cell morphology & locomotion
• vinculin binds to alpha-actinin, paxillin, tensin, actin filaments & phospholipids
• binding to actin occurs via the + end of F-actin
• binding of vinculin to alpha-actinin & to talin links F-actin to plasma membrane integrins while capping F-actin
• phosphorylated; on Ser, Thr & Tyr
• phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions & cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques
• exhibits self-association properties
• interacts with NRAP & SORBS1 (putative)
• interacts with TLN1
• interacts with SYNM

Structure

• aceylated; mainly by myristic acid, but also small amount of palmitic acid (putative)
• belongs to the vinculin/alpha-catenin family

Compartment

• cytoplasm, cytoskeleton
• cell junction, adherens junction
• cell membrane; peripheral membrane protein cytoplasmic side
• cytoplasmic face of adhesion plaques

Alternative splicing

named isoforms=3

Expression

Metavinculin is muscle-specific

Pathology

• defects in VCL are associated with dilated cardiomyopathy type 1W

Laboratory

• vinculin Ab in serum

More general terms

• cytoskeletal protein
• phosphoprotein

More specific terms

• meta-vinculin

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=7414
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:7414
• OMIM: https://mirror.omim.org/entry/193065
• OMIM: https://mirror.omim.org/entry/611407
• UniProt: http://www.uniprot.org/uniprot/P18206.html