src proto-oncogene protein
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Function
- associates with integrin-dependent cytoskeletal complexes, the focal adhesion kinase (FAK) & paxillin
- phosphorylated Y527 binds to SH2 domain, masks kinase domain, & inactives the enzyme
- dephosphorylation of Y527 activates pp60c-src
- substrate specificity of src matches the binding preference of its SH2-domain[5]
- SH2 binding preference as pTyr-Glu-Glu-Ile[5] vs RLIEDNE[Y*]TARQGAK[4]
- interacts with DDEF1/ASAP1 via its SH3 domain
- interacts with CCPG1 (putative)
- interacts with CDCP1, PELP1, TGFB1I1 & TOM1L2
- interacts with the cytoplasmic domain of MUC1, phosphorylates it & increases binding of MUC1 with beta-catenin
- phosphorylated on Tyr-530 by c-Src kinase (CSK)
- phosphorylated form is termed pp60c-src
- phosphorylated tail interacts with the SH2 domain & represses kinase activity
Structure
- belongs to the protein kinase superfamily, Tyr protein kinase family, SRC subfamily
- contains 1 protein kinase domain
- contains 1 SH2 domain
- contains 1 SH3 domain
Compartment
- cytoplasm & plasma membrane (internal face)
Alternative splicing
named isoforms=2
Expression
- expressed in NK-cells, mast cells, platelets
- expressed in transformed T-cells (cytoplasm)
- not expressed in normal T-cells
More general terms
More specific terms
References
- ↑ Einspahr et al, Protein tyrosine phosphorylation and p56lck modification in IL-2 or phorbol ester-activated human natural killer cells. J Immunol 1990 Sep 1;145(5):1490-7 PMID: https://www.ncbi.nlm.nih.gov/pubmed/1696599
- ↑ Bagrodia S, Chackalaparampil I, Kmiecik TE, Shalloway D. Altered tyrosine 527 phosphorylation and mitotic activation of p60c-src. Nature. 1991 Jan 10;349(6305):172-5. PMID: https://www.ncbi.nlm.nih.gov/pubmed/1702522
- ↑ Bolen JB, Rowley RB, Spana C, Tsygankov AY. The Src family of tyrosine protein kinases in hemopoietic signal transduction. FASEB J. 1992 Dec;6(15):3403-9. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/1281458
- ↑ 4.0 4.1 Kemp BE, Pearson RB. Protein kinase recognition sequence motifs. Trends Biochem Sci. 1990 Sep;15(9):342-6. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/2238044
- ↑ 5.0 5.1 5.2 Pawson T Protein modules and signalling networks Nature 373:573 1995 PMID: https://www.ncbi.nlm.nih.gov/pubmed/7531822
- ↑ Clark EA & Brugge JS Integrins and signal transduction pathways: the road taken. Science 268:233 1995 PMID: https://www.ncbi.nlm.nih.gov/pubmed/7716514
- ↑ UniProt http://www.uniprot.org/uniprot/P12931.html