Cullin-3; CUL-3 (CUL3 KIAA0617)

Function

• core component of multiple cullin-RING-based BCR complex(es) (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination & subsequent proteasomal degradation of target proteins
• scaffold protein that may contribute to catalysis through positioning of substrate & ubiquitin-conjugating enzyme
• E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit & is inhibited by the association of the deneddylated cullin with TIP120A/CAND1 (putative)
• functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component
• BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY & DAXX, & probably GLI2 or GLI3.
• BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes & thereby coordinates faithful mitotic progression & completion of cytokinesis
• involved in ubiquitination of cyclin E & of cyclin D1 (in vitro) thus involved in regulation of G1/S transition
• protein modification; protein ubiquitination
• neddylated
• NEDD8 is required for E3 ubiquitin-protein ligase activity of the BCR complex
• deneddylated via its interaction with the COP9 signalosome (CSN complex)
• forms neddylation-dependent homodimers
• interacts with RBX1, RNF7, CYCE & TIP120A/CAND1
• part of the BCR(SPOP) containing SPOP
• part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 & KLHL13
• part of the BCR(KBTBD10) complex containing KBTBD10.
• part of the BCR(ENC1) complex containing ENC1
• part of a complex consisting of BMI1/PCGF4, CUL3 & SPOP
• part of a complex consisting of H2AFY, CUL3 & SPOP
• interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1L, BTBD1
• part of a complex that contains CUL3, RBX1 & GAN

Structure

belongs to the cullin family

Compartment

nucleus, Golgi

Alternative splicing

named isoforms=3

Expression

widely expressed

More general terms

• cullin
• phosphoprotein

Component of

• BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (KLHL20)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=8452
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:8452
• OMIM: https://mirror.omim.org/entry/603136
• UniProt: http://www.uniprot.org/uniprot/Q13618.html