ADP-ribosylation factor-binding protein GGA1; gamma-adaptin-related protein 1; Golgi-localized, gamma ear-containing, ARF-binding protein 1 (GGA1)
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Function
- plays a role in protein sorting & trafficking between trans-Golgi network (TGN) & endosomes
- mediates ARF-dependent recruitment of clathrin to the TGN & binds ubiquitinated proteins & membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif
- phosphorylated by CK2 & dephosphorylated by PP2A
- phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain & to inhibit recognition of cargo signals
- phosphorylated upon DNA damage, probably by ATM or ATR
- ubiquitinated (putative)
- interacts with NECAP1
- interacts with CNST
- interacts with GGA2 & GGA3
- binds to clathrin & activated ARFs
- interacts with RABEP1 & RABGEF1
- interacts with type-1 membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR & BACE1
- binds CCDC91, P200, SYNRG, EPN4, NECAP2 & AFTPH/aftiphilin
- interacts with TSG101 & UBC
Structure
- monomer
- the VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by 2 Leu (AC-LL motif)
- the GAT domain
- the unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs
- the GAE domain binds accessory proteins regulating GGAs function
- belongs to the GGA protein family
- contains 1 GAE domain
- contains 1 GAT domain
- contains 1 VHS domain
Compartment
- Golgi, trans-Golgi network
- membrane; peripheral membrane, endosome membrane
Alternative splicing
named isoforms=2
Expression
ubiquitously expressed