adaptin ear-binding coat-associated protein 1 (NECAP-1) (NECAP1)

Function

• role in endocytosis (putative)
• interacts with AP1G1 & AP2A1 components of the adapter protein complexes AP-1 & AP-2
• interacts with the GAE domain proteins GGA1, GGA2 & GGA3 (putative)

Structure

• belongs to the NECAP family
• WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs & AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif & the properties of the 2nd residue of the motif itself
• WXXF motif 1, which is preceded by an acidic residue & has a Gly in second position mediates specific interaction with AP-1
• WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2 (putative)

Compartment

• cytoplasmic vesicle
• clathrin-coated vesicle membrane (putative)
• cell membrane
• colocalizes with AP-2 at the plasma membrane (putative)

Alternative splicing

named isoforms=2

More general terms

• other protein

Database

• UniProt: http://www.uniprot.org/uniprot/Q8NC96.html