ADP-ribosylation factor-binding protein GGA2; gamma-adaptin-related protein 2; Golgi-localized, gamma ear-containing, ARF-binding protein 2; VHS domain & ear domain of gamma-adaptin; Vear (GGA2, KIAA1080)

Function

• plays a role in protein sorting & trafficking between the trans-Golgi network (TGN) & endosomes
• mediates the ARF-dependent recruitment of clathrin to the TGN & binds ubiquitinated proteins & membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif
• ubiquitinated (putative)
• interacts with NECAP1, TSG101, UBC & AFTPH/aftiphilin
• interacts with CNST (putative)
• interacts with GGA1 & GGA3
• binds to clathrin & activated ARFs
• binds RABEP1 & RABGEF1
• interacts with the type-1 membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR & BACE1
• binds the accessory proteins CCDC91, P200, SYNRG, EPN4 & NECAP2

Structure

• monomer
• the VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by 2 Leu (AC-LL motif)
• the GAT domain
  • responsible for interaction with ARF- GTP, UBC & RABEP1
  • required for recruitment to the TGN
  • prevents ARF-GTP hydrolysis
• the unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs
• the GAE domain binds accessory proteins regulating GGAs function
• belongs to the GGA protein family
• contains 1 GAE domain
• contains 1 GAT domain
• contains 1 VHS domain

Compartment

• Golgi, trans-Golgi network
• membrane; peripheral membrane, endosome membrane

Expression

ubiquitously expressed

More general terms

• other protein

Additional terms

• ADP-ribosylation factor [ARF] subfamily protein
• gamma adaptin (adaptor-related protein complex, gamma subunit)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=23062
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:23062
• OMIM: https://mirror.omim.org/entry/606005
• UniProt: http://www.uniprot.org/uniprot/Q9UJY4.html