17-hydroxycorticosteroids in 24 hour urine

Indications

• used in conjunction with metyrapone stimulation test

Reference interval

• adult
  • male: 3.0-10.0 mg/day (8.3-27.6 umol/day)
  • female: 2.0-8.0 mg/day (5.5-22.1 umol/day)
  • 2.0-6.5 mg/g of creatinine (0.62-2.03 mmol/mol creatinine)
• child: 1.0-5.6 mg/day (2.8-15.5 mg/day)
• infant: (0-1 year): 0.5-1.0 mg/day (1.4-2.8 umol/day)

Increases

• pharmaceutical agents
  • in-vivo effects
    • ACTH, cortisone acetate, gonadotropins, hydrocortisone, trilostane
  • chemical interferences
    • acetazolamide, cephalothin, cefoxitin, chloral hydrate, chlordiazepoxide, chlorpromazine, colchicine, digitalis, erythromycin, fructose, glutethimide, iodides, ketoprofen, meprobamate, methenamine, methicillin, methyprylon, oleandomycin, paraldehyde, phenothiazines, spironolactone, troleandomycin
• clinical disorders
  • Cushing's syndrome
  • stress or any acute illness
  • obesity
  • pregnancy
  • severe hypertension
  • acromegaly
  • thyrotoxicosis

Decreases

• pharmaceutical agents
  • in-vivo effects
    • corticosteroids, dextropropoxyphene, estrogens, medroxyprogesterone, meperidine, morphine, oral contraceptives, pentazocine, phenytoin
  • chemical interferences
    • carbamazepine, reserpine (high doses), thiazides
• clinical disorders
  • Addison's disease
  • congenital adrenal hyperplasia (adrenogenital syndromes)
  • hypopituitarism
  • hypothyroidism

Methods

• colorimetric (Porter-Silber)

Specimen

• urine, 24 hours
• preserve with boric acid & refrigerate
• stable for at least 2 weeks & for longer periods if frozen at -20 degrees C

Notes

• test has largely been replaced by serum cortisol & urine cortisol levels

More general terms

• 24 hour urine

Additional terms

• 17 hydroxycorticosteroid
• 17-ketogenic steroids in 24 hour urine
• 17-ketosteroids in 24 hour urine
• cortisol in serum
• free cortisol in 24 hour urine
• metyrapone stimulation test

Component of

• 17-hydroxycorticosteroids/creatinine in 24 hour urine