17-ketogenic steroids in 24 hour urine

Indications

• used in conjunction with metyrapone stimulation test

Reference interval

• adult
  • male: 5-23 mg/day (17-80 umol/day)
  • female: 3-15 mg/day (10-52 umol/day)

Clinical significance

the test measures:

• corticosteroids
• their precursors
  • 17-hydroxyprogesterone
  • pregnane triol
• their metabolites
  • cortols
  • cortolones

Increases

• pharmaceutical agents
  • in-vivo effects
    • ACTH, cortisone acetate, hydrocortisone
  • chemical interferences
    • cephalothin, digitoxin, meprobamate, phenothiazines, naproxen (m-dinitrobenzene reagent), penicillin, spironolactone
• clinical disorders
  • Cushing's syndrome
  • congenital adrenal hyperplasia
  • obesity
  • acute illness
  • pregnancy

Decreases

• pharmaceutical agents
  • in-vivo effects
    • ampicillin, dexamethasone, estrogens, phenytoin, oral contraceptives, prednisone, prednisolone
  • chemical interferences
    • chlordiazepoxide, glucose, meglumine
• clinical disorders
  • Addison's disease
  • hypopituitarism
  • hypothyroidism
  • generalized wasting syndrome

Methods

• colorimetric (Zimmerman)

Specimen

• urine, 24 hours
• preserve with boric acid & refrigerate
• stable for at least 2 weeks & for longer periods if frozen at -20 degrees C

Notes

• largely replaced by specific assays of plasma & urine steroids

More general terms

• 24 hour urine
• 17-ketogenic steroids in urine

Additional terms

• 17-hydroxycorticosteroids in 24 hour urine
• 17-ketosteroids in 24 hour urine
• metyrapone stimulation test