SH3 domain-containing RING finger protein 1 (plenty of SH3s, protein POSH, SH3 multiple domains protein 2, RING finger protein 142, SH3RF1, KIAA1494, POSH, RNF142, SH3MD2)
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Function
- acts as a scaffold protein
- contributes to Rac-induced signal transduction via JNKs (MAPK8 & MAPK9) activation & induces apoptosis
- within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 & JNKs (MAPK8 & MAPK9)
- may be involved in targeting of HIV-1 to the plasma membrane
- might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N & then transfers it to substrates
- in the absence of an external substrate, it can catalyze self-ubiquitination
- interacts with Rac (GTP-dependent)
- interacts with MAP3K10 & MAP3K11
- interacts with MAPK8IP; interaction leads to PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio
- interacts with SIAH1
- interacts with HERP1
- probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 & AKT2
- phosphorylated at Ser-472 by AKT1 & AKT2.
- when phosphorylated, it has reduced ability to bind Rac
- subject to ubiquitination & proteasomal degradation
Structure
- the RING finger domain is responsible of ubiquitination & proteasomal degradation
- belongs to the SH3RF family
- contains 1 RING-type Zn+2 finger
- contains 6 SH3 domains
Compartment
- cytoplasm, perinuclear region
- cell projection, lamellipodium
- Golgi, trans-Golgi network
- colocalizes, with AKT2, in lamellipodia (putative)
- colocalizes, with HERP1, in trans-Golgi network
Alternative splicing
named isoforms=3