mitogen-activated protein kinase 9 (c-jun NH2 terminal kinase 2, JNK2, JNK55, MAPK9, PRKM9)
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Function
- responds to activation by environmental stress & pro- inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun & ATF2 & thus regulates AP-1 transcriptional activity
- in T-cells, JNK1 & JNK2 are required for polarized differentiation of T-helper cells into Th1 cells
- JNK2 isoforms display different binding patterns:
- no correlation between binding & phosphorylation, which is achieved at about the same efficiency by all isoforms
- JUNB is not a substrate for JNK2 alpha-2
- JUND binds only weakly to JNK2 alpha-2
- binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 & SPAG9/MAPK8IP4/JIP-4
- these proteins also bind other components of the JNK signaling pathway
- activated by Thr & Tyr phosphorylation by either of 2 dual specificity kinases, MAP2K4 & MAP2K7
- inhibited by dual specificity phosphatases, such as DUSP1
- dually phosphorylated on Thr-183 & Tyr-185, which activates the enzyme
- autophosphorylated in vitro
Structure
- TXY motif contains Thr & Tyr whose phosphorylation activates the MAP kinases
- belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
- contains 1 protein kinase domain
Alternative splicing
named isoforms=4
More general terms
References
- ↑ Sun H, Tonks NK, Bar-Sagi D. Inhibition of Ras-induced DNA synthesis by expression of the phosphatase MKP-1. Science. 1994 Oct 14;266(5183):285-8. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7939666
- ↑ Kyriakis JM, Banerjee P, Nikolakaki E, Dai T, Rubie EA, Ahmad MF, Avruch J, Woodgett JR. The stress-activated protein kinase subfamily of c-Jun kinases. Nature. 1994 May 12;369(6476):156-60. PMID: https://www.ncbi.nlm.nih.gov/pubmed/8177321
- ↑ Atlas of genetics & cytogenetics in oncology & haematology http://atlasgeneticsoncology.org/genes/JNK2ID426.html
- ↑ UniProt http://www.uniprot.org/uniprot/P45984.html