ubiquitin-conjugating enzyme E2 N; Bendless-like ubiquitin-conjugating enzyme; Ubc13; ubiquitin carrier protein N; ubiquitin-protein ligase N (UBE2N, BLU)
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Function
- the UBE2V1-UBE2N & UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys-63-linked polyubiquitin chains
- this type of polyubiquitination does not lead to protein degradation by the proteasome
- mediates transcriptional activation of target genes
- role in the control of progress through the cell cycle & differentiation
- role in error-free DNA repair; contributes to survival of cells after DNA damage
- acts together with the E3 ligases, HLTF & SHPRH, in the Lys-63-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair
- appears to act together with E3 ligase RNF5 in the Lys-63-linked polyubiquitination of JKAMP thus regulating JKAMP function by decreasing its association with components of the proteasome & ERAD
- protein modification; protein ubiquitination
- conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2
- heterodimer with UBE2V2
- interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain)
- complex has a specific Lys-63-linked polyubiquitination activity
- interacts with RNF8 & RNF168
- interacts with RNF11
- interacts with the E3 ligases, HLTF & SHPRH
- interactions promote Lys-63-linked polyubiquitination of PCNA upon genotoxic stress & lead to DNA repair
- interacts with ARIH2 (via RING-type 2)
Structure
- belongs to the ubiquitin-conjugating enzyme family