E3 ubiquitin-protein ligase SHPRH (SNF2, histone-linker, PHD & RING finger domain-containing helicase; SHPRH ;KIAA2023)

Function

• E3 ubiquitin-protein ligase involved in DNA repair
• upon genotoxic stress, accepts ubiquitin from UBE2N-UBE2V2 E2 complex & transfers it to Lys-164 of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting formation of non-canonical poly-ubiquitin chains linked through Lys-63
• ubiquitin conjugation, 3rd step.
• interacts with PCNA, UBE2N & RAD18

Structure

• homodimer
• belongs to the SNF2/RAD54 helicase family
• contains 1 helicase ATP-binding domain
• contains 1 helicase C-terminal domain
• contains 1 histone H1 domain
• contains 1 PHD-type zinc finger
• contains 1 RING-type zinc finger
  • mediates E3 ubiquitin ligase activity

Alternative splicing

named isoforms=5

Expression

broadly expressed

More general terms

• E3 ubiquitin ligase
• ring finger protein

Database

• OMIM: https://mirror.omim.org/entry/608048
• UniProt: http://www.uniprot.org/uniprot/Q149N8.html