T-lymphoma invasion & metastasis-inducing protein 2 (TIAM-2, SIF & TIAM1-like exchange factor, TIAM2, KIAA2016, STEF)

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Function

modulates the activity of RHO-like proteins & connects extracellular signals to cytoskeletal activities
acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases & activates them
mediates extracellular laminin signals to activate Rac1, contributing to neurite growth
involved in lamellipodial formation & advancement of the growth cone of embryonic hippocampal neurons
promotes migration of neurons in the cerebral cortex
when overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (putative)
activates specifically RAC1, but not CDC42 & RHOA
interacts with MAP1A, MAP1B, PARP1 & YWHAE
phosphorylated on serine & threonine residues
phosphorylated on Thr-1648 by Rho-kinase
phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 & YWHAE & reduces its ability to promote neurite growth (putative)

PH 1 domain & amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization
PH 1 & TSS domains are necessary for Rac1 activity
PH 2 domain is engaged in the enhancement of catalytic activity of the adjacent DH domain
PH 1, TSS & DH domains are necessary to induce neurite-like structure
belongs to the TIAM family
contains 1 DH (DBL-homology) domain
contains 1 PDZ (DHR) domain
contains 2 PH domains
contains 1 RBD (Ras-binding) domain