DNA repair protein RAD51 homolog 1; hRAD51; HsRAD51 (RAD51, RAD51A, RECA)

Function

• role in a common DNA damage response pathway associated with activation of homologous recombination & double-strand break repair
• binds to single-stranded DNA & double-stranded DNA & exhibits DNA-dependent ATPase activity
• unwinds duplex DNA & forms helical nucleoprotein filaments
• phosphorylated
• phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage & promote DNA repair by homologous recombination
• interacts with BRCA1, BRCA2 & either directly or indirectly with p53
• interacts with XRCC3, RAD54L & RAD54B
• part of a complex with RAD51C & RAD51B
• interacts with RAD51AP1 & RAD51AP2
• interacts with CHEK1/CHK1; this may require prior phosphorylation of CHEK1
• interacts with the MND1-PSMC3IP
• heterodimer (putative)
• interacts with OBFC2B
• appears to be involved in surveillance of chromosomes for double-strand breaks

Structure

• belongs to the recA family, RAD51 subfamily
• contains 1 HhH domain

Compartment

• nucleus
• colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage

Alternative splicing

named isoforms=2

Expression

• highly expressed in testis & thymus > small intestine, placenta, colon, pancreas & ovary > breast

Pathology

• defects in RAD51 are associated with breast cancer

More general terms

• DNA repair protein Rad51 homolog
• oligomerizing protein
• phosphoprotein

Component of

• BRCC holoenzyme complex

Database

• UniProt: http://www.uniprot.org/uniprot/Q06609.html
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=5888
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:5888
• OMIM: https://mirror.omim.org/entry/114480
• OMIM: https://mirror.omim.org/entry/179617