protein arginine N-methyltransferase 5; 72 kD ICln-binding protein; histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs (PRMT5, HRMT1L5, IBP72, JBP1, SKB1)

Function

• protein arginine N-methyltransferase
• catalyzes formation of omega-N monomethylarginine (MMA) & symmetrical dimethylarginine (sDMA), with a preference for formation of MMA
• specifically mediates symmetrical dimethylation of Arg in the small nuclear ribonucleoproteins Sm-D1 (SNRPD1) & Sm-D3 (SNRPD3); methylation is required for assembly & biogenesis of snRNP core particles
  • role in the assembly of snRNP core particles
• methylates SUPT5H.
• mono- & dimethylates Arg of myelin basic protein in vitro
• may play a role in cytokine-activated transduction pathways
• negatively regulates cyclin E1 promoter activity & cellular proliferation
• may regulate the SUPT5H transcriptional elongation properties
• may be part of a pathway connected to a chloride current, possibly through cytoskeletal rearrangement
• methylates histone H2A & H4 Arg-3 during germ cell development
• methylates histone H3 Arg-8, which may repress transcription
• methylates the Piwi proteins (PIWIL1, PIWIL2 & PIWIL4)
  • methylation of Piwi proteins is required for interaction with Tudor domain-containing proteins & subsequent localization to the meiotic nuage
• methylates RPS10
• disulfide bonds & non-covalent association mediate homooligomer formation
• forms, at least, homodimers & homotetramers
• interacts with PRDM1 (putative)
• component of the methylosome
• component of a high molecular weight E2F-pocket protein complex, (cyclin E1 repressor complex)
• interacts with Sm proteins, JAK2, SSTR1 & SUPT5H
• associates with SWI/SNF remodeling complexes containing SMARCA2 & SMARCA4
• interacts with LSM11, PRMT7 & SNRPD3
• interacts with COPR5/C17orf79, promoting its recruitment on histone H4.
• interacts with RPS10

S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone]

Structure

• belongs to the protein arginine N-methyltransferasefamily

Compartment

cytoplasm. nucleus

Alternative splicing

• named isoforms=1
• several isoforms may be produced

Expression

ubiquitous

More general terms

• membrane protein
• protein arginine N-methyltransferase (PRMT)

Component of

• methylosome

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=10419
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:10419
• OMIM: https://mirror.omim.org/entry/604045
• UniProt: http://www.uniprot.org/uniprot/O14744.html