docking protein 1; downstream of tyrosine kinase 1; p62(dok); pp62 (DOK1)

Introduction

Docking proteins interact with receptor tyrosine kinases & mediate particular biological responses.

Function

• negative regulator of the insulin signaling pathway
• constitutively Tyr-phosphorylated
• modulates integrin activation by competing with talin for the same binding site on ITGB3
• phosphorylated by TEC (putative)
• phosphorylated by LYN
• phosphorylated on Tyr by the insulin receptor results in negative regulation of the insulin signaling pathway
• interacts with ABL1 (putative)
• interacts with RasGAP & INPP5D/SHIP1
• interacts directly with phosphorylated ITGB3

Structure

• the PTB domain mediates receptor interaction
• belongs to the DOK family, type A subfamily
• contains 1 IRS-type PTB domain
• contains 1 PH domain
• isoform 3 contains a N-acetylmethionine at position 1

Compartment

• isoform 1: cytoplasm
• isoform 3: cytoplasm, perinuclear region

Alternative splicing

named isoforms=3

Alternative initiation

• isoform 3: alternative initiation at Met-140 of isoform 1, acetylated on Met-1

Expression

• expressed in pancreas, heart, leukocyte & spleen
• expressed in both resting & activated peripheral blood T-cells

More general terms

• phosphoprotein
• docking protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=1796
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:1796
• OMIM: https://mirror.omim.org/entry/602919
• UniProt: http://www.uniprot.org/uniprot/Q99704.html