transformation/transcription domain-associated protein; 350/400 kD PCAF-associated factor; PAF350/400; STAF40; tra1 homolog (TRRAP, PAF400)

Function

• adapter protein
• essential for early development
• required for the mitotic checkpoint & normal cell cycle progression
• found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation
• component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 & H2A
• role in MYC transcription activation, & participates in cell transformation by MYC
• required for p53/TP53-, E2F1- & E2F4-mediated transcription activation
• probably acts by linking transcription factors such as MYC or E2F1 to HAT complexes such as STAGA thus allowing transcription activation
• probably not required in the steps following histone acetylation in processes of transcription activation
• may be required for the mitotic checkpoint & normal cell cycle progression
• interacts with MYC, E2F1 & E2F4 transcription factors
• interacts directly with p53/TP53
• interacts with GCN5L2
• component of various HAT complexes
• component of the PCAF complex
• component of the TFTC-HAT complex
• component of the NuA4 histone acetyltransferase complex
• component of the STAGA complex
• component of the BAF53 complex

Structure

• the PI3K/PI4K domain is required for the recruitment of HAT complexes, & the MYC-dependent transactivation
• although strongly related to the PI3/PI4-kinase family, TRRAP lacks typical motifs that constitute the catalytic site of PI3/PI4-kinase proteins, & lacks such activity
• belongs to the PI3/PI4-kinase family, TRA1 subfamily
• contains 1 FAT domain
• contains 1 FATC domain
• contains 1 PI3K/PI4K domain

Compartment

nucleus

Alternative splicing

named isoforms=2

Pathology

• involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes

More general terms

• nuclear protein
• phosphoprotein

Component of

• PCAF complex; coactivator for p53/TP53-dependent transcriptional activation; PCAF histone acetylase complex
• STAGA transcription coactivator-HAT complex
• BAF53 complex

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=8295
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:8295
• OMIM: https://mirror.omim.org/entry/603015
• UniProt: http://www.uniprot.org/uniprot/Q9Y4A5.html