tripartite motif-containing protein 28; transcription intermediary factor 1-beta; TIF1-beta; nuclear corepressor KAP-1; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96 (TRIM28, KAP1, RNF96, TIF1B)
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Function
- forms a complex with a KRAB-domain transcription factor & increases the efficiency of KRAB-mediated repression
- silences transcription through an interaction with HP1 proteins
- acts as a corepressor of transcription for the KRAB Zn+2 finger proteins & as a moderator of the repression activity
- may play a role as a coactivator for CEBPB & NR3C1 in the transcriptional activation of the alpha-1-acid glycoprotein gene
- phosphorylated upon DNA damage, probably by ATM or ATR
- associated with HP1 alpha (CBX5), beta (CBX1) & gamma (CBX3) in interphase nuclei (putative)
- interacts with ZNF382; enhances ZNF382 transcriptional repressor activity
- interacts with CEBPB & NR3C1 (putative)
- interacts with NCOR1 & CHD3
- interacts with SETDB1, ZFP53 & ZFP68
- interacts with CBX5 via the PxVxL motif
- interacts with ZNF256
Structure
- the HP1 box is both necessary & sufficient for HP1 binding
- the RING finger domain & the B-box domains mediate interaction with CEBPB
- the PHD domain enhances the CEBPB transcriptional activity (putative)
- contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif
- required for interaction with chromoshadow domains
- requires additional residues -7, -6, +4 & +5 of the central Val which contact the chromoshadow domain
- belongs to the TRIM/RBCC family
- contains 2 B box-type Zn+2 fingers
- contains 1 bromo domain
- contains 1 PHD-type Zn+2 finger
- contains 1 RING-type Zn+2 finger
Compartment
- nucleus (putative)
- associated with centromeric heterochromatin during cell differentiation through CBX1 (putative)
Alternative splicing
named isoforms=2