histone-H3.2 (H3/m, H3/o, HIST2H3A)

Function

• expressed during S phase
• expression decreases as cell division slows down during differentiation.
• acetylation is generally linked to gene activation.
  • acetylation on Lys-9 impairs methylation at Arg-8
  • acetylation on Lys-18 & Lys-23 favors methylation at Arg-17
• citrullination at Arg-8 &/or Arg-17 by PADI4 impairs methylation & represses transcription
• asymmetric dimethylation at Arg-17 by CARM1 is linked to gene activation
• symmetric dimethylation at Arg-8 by PRMT5 is linked to gene repression
• methylation at Lys-4, Lys-36 & Lys-79 are linked to gene activation
  • methylation at Lys-4 facilitates subsequent acetylation of H3 & H4
• methylation at Lys-79 is associated with DNA double-strand break responses & is a specific target for TP53BP1
• methylation at Lys-9 & Lys-27 are linked to gene repression
• methylation at Lys-9 is a specific target for HP1 proteins (CBX1, CBX3 & CBX5) & prevents subsequent phosphorylation at Ser-10 & acetylation of H3 & H4
• methylation at Lys-4 & Lys-79 require preliminary monoubiquitination of H2B at Lys-120
• methylation at Lys-9 & Lys-27 are enriched in inactive X chromosome chromatin
• phosphorylated at Thr-3 by GSG2/haspin during prophase & dephosphorylated during anaphase
• at centromeres, specifically phosphorylated at Thr-11 from prophase to early anaphase
• phosphorylated at Ser-10 during all of mitosis
  • linked to gene activation
  • prevents methylation at Lys-9
  • facilitates acetylation of H3 & H4
• phosphorylated at Ser-28 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation
• ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation
  • may weaken interaction between histones & DNA & facilitate DNA accessibility to repair proteins

Structure

belongs to the histone H3 family

Compartment

nucleus

Expression

• expressed during S phase
• expression strongly decreases as cell division slows down during the process of differentiation

More general terms

• histone-H3
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=126961
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=333932
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=653604
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:126961
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:333932
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:653604
• OMIM: https://mirror.omim.org/entry/142780
• UniProt: http://www.uniprot.org/uniprot/Q71DI3.html