E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; Gu-binding protein; GBP; protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein (PIAS1, DDXBP1)
Jump to navigation
Jump to search
Function
- E3-type small ubiquitin-like modifier (SUMO) ligase
- stabilizes interaction between UBE2I & substrate
- SUMO-tethering factor
- role in transcriptional coregulation in cellular pathways
- STAT pathway
- p53 pathway
- steroid hormone signaling pathway
- effects of transcriptional coregulation may be transactivation or silencing, depending upon context
- protein modification; protein sumoylation
- sumoylated
- binds SUMO1 & UBE2I
- interacts with AR, AXIN1, CSRP2, JUN, MDM2, NCOA2, TP53, RNA helicase 2 & STAT1 dimers, following IFN-alpha stimulation
- interacts with SP3, preferentially when SUMO-modified
- preferentially binds AT-rich DNA sequences, known as matrix attachment regions or scaffold attachment regions (putative)
- interacts with PLAG1
- interacts with KLF8; interaction results in SUMO ligation & repression of KLF8 transcriptional activity & of its cell cycle progression into G1 phase
- interacts with SATB2
Structure
- the LXXLL motif is a transcriptional coregulator signature
- the SP-RING-type domain is required for promoting EKLF sumoylation (putative)
- belongs to the PIAS family
- contains 1 PINIT domain
- contains 1 SAP domain
- contains 1 SP-RING-type Zn+2 finger
Compartment
- nuclear speckle
- interaction with CSRP2 may induce a partial redistribution along the cytoskeleton
Expression
- expressed in numerous tissues
- highest expression in testis
More general terms
Additional terms
- DEAD/H ATP helicase or DEAD/H box
- interferon-gamma activated factor; gamma-activated factor; signal transducer & activator of transcription-1 (STAT1)