Krueppel-like factor 1; erythroid krueppel-like transcription factor; EKLF (KLF1, EKLF)
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Function
- transcription regulator of erythrocyte development
- binds to the CACCC box in the beta-globin gene promoter & activates transcription
- probably serves as a general switch factor for erythroid development
- sumoylated; when sumoylated
- acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta
- represses megakaryocytic differentiation
- promotes interaction with the CDH4 subunit of the NuRD repression complex
- sumoylation is promoted by PIAS1
- acetylated; can be acetylated on both Lys-274 & Lys-288
- acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (putative)
- phosphorylated primarily on Ser in the transactivation domain.
- phosphorylation on Thr-23 is critical for the transactivation activity (putative)
- interacts with CBP & EP300; the interactions enhance the transactivation activity
- interacts with PCAF; the interaction
- does not acetylate EKLF
- inhibits its transactivation activity
Structure
- belongs to the krueppel C2H2-type Zn+2-finger protein family
- contains 3 C2H2-type Zn+2 fingers
Compartment
- nucleus
- colocalizes with SUMO1 in nuclear speckles (putative)
Expression
- expression restricted to adult bone marrow & fetal liver
- not expressed in myeloid nor lymphoid cell lines
Pathology
- defects in KLF1 are the cause of blood group-Lutheran inhibitor