Rho guanine nucleotide exchange factor 2; guanine nucleotide exchange factor H1; GEF-H1; microtubule-regulated Rho-GEF; proliferating cell nucleolar antigen p40 (ARHGEF2, KIAA0651, LFP40)

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Function

activates Rho-GTPases by promoting the exchange of GDP for GTP
may be involved in
- epithelial barrier permeability
- cell motility & polarization
- dendritic spine morphology
- antigen presentation
- leukemic cell differentiation
- cell cycle regulation
- cancer
binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases
may stimulate the cortical activity of Rac
inactive toward CDC42, TC10, or Ras-GTPases
forms an intracellular sensing system along with NOD1 for detection of microbial effectors during cell invasion by pathogens
required for RHOA & RIP2 dependent NF-kappaB signaling upon S flexneri cell invasion
role in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 independent of its GEF activity
role in activation of NF-kappaB by Shigella effector proteins (IpgB2 & OspB) which requires its GEF activity & activation of RhoA
phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules & inhibition of its activity
phosphorylated by AURKA & CDK1 during mitosis, which negatively regulates its activity
phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity
phosphorylation by PAK4 releases GEF-H1 from the microtubules
interacts with 14-3-3 zeta when phosphorylated at Ser-886
interacts with the kinases PAK4, AURKA & MAPK1
interacts with RHOA & RAC1
interacts with NOD1
interacts (via the N-terminal Zn+2 finger) with CAPN6 (via domain II) (putative)