protein arginine N-methyltransferase 2; histone-arginine N-methyltransferase PRMT2 (PRMT2, HMT1, HRMT1L1)

Function

• protein arginine N-methyltransferase
• methylates the guanidino nitrogens of Arg in proteins including STAT3, FBL, histone H4
• acts as a coactivator (with NCOA2) of androgen receptor- mediated transactivation
• acts as a coactivator (with estrogen) of estrogen receptor- mediated transactivation
• enhances PGR, PPARG, & RARA-mediated transactivation
• may inhibit NF-kappa-B transcription & promote apoptosis
• represses E2F1 transcriptional activity (RB1-dependent)
• may be involved in growth regulation
• self-associates
• interacts with RB1 & E2F1
• interacts with NCOA6 coactivator
• interacts (via SH3 domain) with PRMT8
• interacts with AR
• interacts with NFKBIA
• interacts with ESR1, ESR2, PGR, PPARG, RARA, RXRA & THRB
• interacts with HNRNPUL1

S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone]

Kinetic parameters:

• KM=2.6 uM for AdoMet
• KM=3.3 uM for H4
• Vmax=1.4 nmol/min/mg enzyme toward AdoMet
• Vmax=1.5 nmol/min/mg enzyme toward H4

Structure

• belongs to the protein arginine N-methyltransferase family
• contains 1 SH3 domain

Compartment

• cytoplasm, nucleus
• translocates from cytoplasm to nucleus, after hormone exposure

Expression

• widely expressed
• highly expressed in androgen target organs including heart, prostate, skeletal muscle, ovary & spinal cord

More general terms

• protein arginine N-methyltransferase (PRMT)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=3275
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:3275
• OMIM: https://mirror.omim.org/entry/601961
• UniProt: http://www.uniprot.org/uniprot/P55345.html