protein arginine N-methyltransferase 8; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 (PRMT8, HRMT1L4)

Function

• membrane-associated protein arginine N-methyltransferase
• catalyzes formation of omega-N monomethylarginine (MMA) & asymmetrical dimethylarginine (aDMA)
• able to mono- & dimethylate EWS protein;
• role toward EWS remains unclear as it still interacts with fully methylated EWS
• homodimers & heterodimers with PRMT1 or PRMT2, recruiting PRMT1 to the cell membrane
• interacts with PRMT2 & FYN (via the SH3 domain)
• interacts with EWS; independently of EWS methylation status

Kinetic parameters:

• KM=1.3 uM for GRGGFGGRGGFRGGRGG-NH2

Structure

• the SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 & FYN, possibly leading to displace the N-terminal domain & activate the protein the N-terminal region (1-60) inhibits the enzymatic activity
• belongs to the protein arginine N-methyltransferase family, PRMT8 subfamily

Compartment

• cell membrane; lipid-anchor; cytoplasmic side

Alternative splicing

named isoforms=2

Expression

brain-specific

More general terms

• protein arginine N-methyltransferase (PRMT)
• myristoylated protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=56341
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:56341
• OMIM: https://mirror.omim.org/entry/610086
• UniProt: http://www.uniprot.org/uniprot/Q9NR22.html