ubiquitin-conjugating enzyme E2 L3; L-UBC; UbcH7; ubiquitin carrier protein L3; ubiquitin-conjugating enzyme E2-F1; ubiquitin-protein ligase L3 (UBE2L3, UBE2L1, UBCE7, UBCH7)
Jump to navigation
Jump to search
Function
- ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type & RBR family E3 ubiquitin-protein ligases
- does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 & ARIH1, that function like function like RING-HECT hybrids
- accepts ubiquitin from the E1 complex & catalyzes its covalent attachment to other proteins
- in vitro catalyzes Lys-11-linked polyubiquitination
- involved in the selective degradation of short-lived & abnormal proteins
- down-regulated during the S-phase
- role in progression through the cell cycle
- regulates transcriptional activity of genes encoding nuclear hormone receptors
- may play a role in myelopoiesis
- protein modification; protein ubiquitination
- ubiquitinated
- alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation
- interacts with PARK2
- role in ubiquitination & degradation of misfolded proteins
- interacts with UBE3A
- interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B & RNF144B
- interacts with ARIH1
- interacts with ARIH2 (via RING-type 1)
- interacts with NCOA1
- interaction regulates transcriptional activity of progesterone receptor gene
- may interact with NR3C1
Structure
- in contrast to other ubiquitin-conjugating enzymes E2, residues essential for lysine reactivity are absent:
- Pro & His are present instead of Asp in positions 88 & 119, respectively
- belongs to the ubiquitin-conjugating enzyme family
Compartment
Expression
- ubiquitous
- highest expression in testis
Pathology
- used by the papillomavirus HPV-16 E6 protein to ubiquitinate p53/TP53
Notes
- 2 genes for ubiquitin-conjugating proteins UBE2L1/UBE2L3 are identical except that the E2L1 gene is intronless, while the E2L3 coding sequence is interrupted by 3 introns[1]
- UBE2L1, UBE2L2 & UBE2L4 are most likely pseudogenes & the only expressed member of this subfamily seems to be UBE2L3[3]
More general terms
References
- ↑ 1.0 1.1 UniProt http://www.uniprot.org/uniprot/P68036.html
- ↑ OMIM https://mirror.omim.org/entry/600012
- ↑ 3.0 3.1 Ardley HC et al Promoter analysis of the human ubiquitin-conjugating enzyme gene family UBE2L1-4, including UBE2L3 which encodes UbcH7 Biochim Biophys Acta. 2000 Apr 25;1491(1-3):57-64. PMID: https://www.ncbi.nlm.nih.gov/pubmed/10760570