notch-2 (Drosophila) homolog protein (NOTCH2)

^[1]^[2]^[3]

Function

functions as a receptor for membrane-bound ligands Jagged1, Jagged2 & delta1 to regulate cell-fate determination
upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa & activates genes of the enhancer of split locus
affects implementation of differentiation, proliferation & apoptotic programs (putative)
synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form
cleavage results in a C-terminal fragment N(TM) & a N-terminal fragment N(EC)
following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT)
this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (putative)
phosphorylated (putative)
heterodimer of a C-terminal fragment N(TM) & an N-terminal fragment N(EC) which are probably linked by disulfide bonds (putative)
interacts with MAML1, MAML2 & MAML3 which act as transcriptional coactivators for NOTCH2