M-phase inducer phosphatase-1 (dual-specificity phosphatase Cdc25A, CDC25A)

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Function

tyrosine protein phosphatase
dosage-dependent inducer of mitotic progression
directly dephosphorylates CDC2 & stimulates its kinase activity
dephosphorylates CDK2 in complex with cyclin E, in vitro
stimulated by B-type cyclins
interacts with CCNB1/cyclin B
interacts with YWHAE/14-3-3 epsilon when phosphorylated
interacts with CUL1 specifically when CUL1 is neddylated & active
interacts with BTRC/BTRCP1 & FBXW11/BTRCP2
interactions with CUL1, BTRC & FBXW11 are enhanced upon DNA damage
phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 & Thr-507 during checkpoint mediated cell cycle arrest
phosphorylated by CHEK2 on Ser-124, Ser-279, & Ser-293 during checkpoint mediated cell cycle arrest
phosphorylation on Ser-178 & Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A
phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 & Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A
ubiquitinated
association with the F-box proteins BTRC & FBXW11 targets the protein for ubiquitination by CUL1 & proteolysis by the ubiquitin-dependent proteasome pathway

protein tyrosine phosphate + H2O
          <-->
protein tyrosine + phosphate

phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated; phosphorylation sites at Ser-79 & Ser-82 appear to be essential for this interaction
belongs to the MPI phosphatase family
contains 1 rhodanese domain