cyclin-dependent kinase-2 (CDK2)
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Function
- role in the control of the cell cycle
- interacts with cyclins A, B1, B3, D, or E
- expressed constitutively throughout the cell cycle
- activity of CDK2 is maximal during S phase & G2
- CDK2/cyclin-E is maximally activated a few hours before onset of DNA synthesis
- found in a complex with CABLES1, CCNA1 & CCNE1
- interacts with CABLES1 (putative)
- interacts with UHRF2
- part of a complex consisting of UHRF2, CDK2 & CCNE1
- interacts with the speedy/Ringo proteins SPDYA & SPDYC
- found in a complex with both SPDYA & CDKN1B/KIP1
- phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme
- phosphorylation at Thr-160 activates enzyme
- association of CDK2 with cyclins A or E with subsequent dephosphorylation of Tyr-15 & Thr-14 on CDK2 by cdc25 phosphatase & an independently regulated phosphorylation of Thr-160 activates CDK2
- CDK2 activity is required for DNA synthesis & is responsible for the phosphorylation of Rb protein & the modulation of DNA-bound replication protein A
- CDK2 is the G1 kinase of fibroblasts
Structure
- belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family
- CDC2/CDKX subfamily contains 1 protein kinase domain
More general terms
Component of
References
- ↑ Dulie V, Lees E, Reed SI. Association of human cyclin E with a periodic G1-S phase protein kinase. Science. 1992 Sep 25;257(5078):1958-61. PMID: https://www.ncbi.nlm.nih.gov/pubmed/1329201
- ↑ De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO, Kim SH. Crystal structure of cyclin-dependent kinase 2. Nature. 1993 Jun 17;363(6430):595-602. PMID: https://www.ncbi.nlm.nih.gov/pubmed/8510751 {crystal}
- ↑ Morgan DO. Principles of CDK regulation. Nature. 1995 Mar 9;374(6518):131-4. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/7877684
- ↑ UniProt http://www.uniprot.org/uniprot/P24941.html