protein kinase C-zeta (PRKCZ, PKC2)

Function

• Ca+2-independent, phospholipid-dependent,
• serine/threonine kinase
• activated by diacylglycerol
• activated by ceramide
• subunit of a quaternary complex that plays a central role in epithelial cell polarization
• forms a ternary complex with SQSTM1 & KCNAB2
• forms another ternary complex with SQSTM1 & GABRR3
• forms a complex with SQSTM1 & MAP2K5 (putative)
• interacts with PARD6A, PARD6B, PARD6G & SQSTM1
• part of a complex with PARD3, PARD6A or PARD6B or PARD6G & CDC42 or RAC1
• interacts with CENTA1
• forms a ternary complex with SQSTM1 & PAWR
• interacts directly with SQSTM1 (probable)
• interacts with IKBKB
• phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator
• 2 specific sites
  • Thr-410 (activation loop of the kinase domain)
  • Thr-560 (turn motif), need to be phosphorylated for its full activation

Structure

• OPR domain mediates mutually exclusive interactions with SQSTM1 & PARD6B
• C1 domain does not bind the diacylglycerol (DAG)
• belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
• contains 1 AGC-kinase C-terminal domain
• contains 1 OPR domain
• contains 1 phorbol-ester/DAG-type Zn+2 finger
• contains 1 protein kinase domain

Compartment

• cytoplasm, endosome
• in the retina, localizes in the terminals of the rod bipolar cells
• associates with endosomes

Expression

expressed in brain > lung, kidney & testis

More general terms

• protein kinase C type-B

Database

• UniProt: http://www.uniprot.org/uniprot/Q05513.html
• OMIM: https://mirror.omim.org/entry/176982
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=5590