centaurin-beta-1; Arf-GAP with coiled-coil, ANK repeat & PH domain-containing protein 1; Cnt-b1 (ACAP1, CENTB1, KIAA0050)
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Function
- GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network & cell surface
- GAP activity stimulated by phosphatidylinositol-4,5-bisphosphate (PIP2) & phosphatidic acid
- phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface & ITGB1-dependent cell migration
- interacts with GTP-bound ARF6
- interacts with third cytoplasmic loop of SLC2A4/GLUT4
- interacts with CLTC
- interacts with GULP1
- forms a complex with GDP-bound ARF6 & GULP1
Structure
- PH domain binds phospholipids including
- PH domain may mediate ACAP1-binding to PIP2 or PIP3 containing membranes
- contains 3 ANK repeats
- contains 1 Arf-GAP domain
- contains 1 BAR domain
- contains 1 PH domain
Expression
Notes
- cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes & inhibition of stimulation- dependent cell migration
- cells with reduced levels of ACAP1 or AKT1 & AKT2 show inhibition of stimulation-dependent cell migration
- cells overexpressing ACAP1 & PIP5K1C show formation of tubular structures derived from endosomal membranes