fibroblast growth factor 2; FGF-2; basic fibroblast growth factor; bFGF; heparin-binding growth factor 2; HBGF-2; prostatropin (FGF2, FGFB)
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Function
- requires heparan sulfate binding for activity
- heparin-binding growth factors are angiogenic agents in vivo & potent mitogens vitro
- binds heparin more strongly than does aFGF
- cell injury may liberate b-FGF
- precursor does NOT contain ER signal sequence
- role in regulation of cell survival, cell division, angiogenesis, cell differentiation & cell migration.
- potent mitogen in vitro
- phosphorylation at Tyr-215 regulates FGF2 unconventional secretion
- interacts with FGFR1, FGFR2, FGFR3 & FGFR4
- affinity between fibroblast growth factors (FGFs) & their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors
- interacts with CSPG4, FGFBP1 & TEC
- found in a complex with FGFBP1, FGF1 & FGF2
- pH dependence: retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees C
- temperature dependence: inactivated after 3 minutes at 60 degrees celsius or 1 minute at 80 degrees C
Structure
- monomer, homodimer
- belongs to the heparin-binding growth factors family
Compartment
- secreted
- exported from cells by an endoplasmic reticulum (ER)/Golgi- independent mechanism
- unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane
- present in the cytoplasm of most cells
Alternative initiation
Named isoforms=4
Expression
- expressed in granulosa & cumulus cells
Pathology
- may play a role in resistanct to cancer chemotherapy[5]
- expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue
Notes
- several N-termini starting at positions 94, 125, 126, 132, 143 & 162 have been identified by direct sequencing
More general terms
References
- ↑ Machida CN et al NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells. Neuron 1989 Jun;2(6):1587-96 PMID: https://www.ncbi.nlm.nih.gov/pubmed/2560648 [
- ↑ Imamura T et al Recovery of mitogenic activity of a growth factor mutant with a nuclear translocation sequence. Science 249:1567 1990 PMID: https://www.ncbi.nlm.nih.gov/pubmed/1699274
- ↑ Henderson B & Blake S Therapeutic potential of cytokine manipulation TIPS 13:145 1992 PMID: https://www.ncbi.nlm.nih.gov/pubmed/1589908
- ↑ Entrez Gene http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=2247
- ↑ 5.0 5.1 Netscape, Dec 3, 2006 sites BBC News
- ↑ UniProt http://www.uniprot.org/uniprot/P09038.html
- ↑ NIEHS-SNPs http://egp.gs.washington.edu/data/fgf2/