transcription factor PU.1; 31 kD-transforming protein (SPI1)
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Function
- binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer
- transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells
- also binds RNA & may modulate pre-mRNA splicing
- binds DNA as a monomer
- interacts with RUNX1 & SPIB
- interacts with CEBPD & NONO (putative)
Structure
- belongs to the ETS family
- contains 1 ETS DNA-binding domain
Compartment
Expression
- normal expression of SPI1 is restricted to B-cells & macrophages
Pathology
- highly expressed in both FV-P & FV-A-induced erythroleukemia cell lines that have undergone rearrangements of the spi-1 gene due to the insertion of SFFV
More general terms
References
- ↑ Bishop JM. Molecular themes in oncogenesis. Cell. 1991 Jan 25;64(2):235-48. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/1988146
- ↑ Macleod K, Leprince D, Stehelin D. The ets gene family. Trends Biochem Sci. 1992 Jul;17(7):251-6. Review. PMID: https://www.ncbi.nlm.nih.gov/pubmed/1502727
- ↑ Pongubala JM, Van Beveren C, Nagulapalli S, Klemsz MJ, McKercher SR, Maki RA, Atchison ML. Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation. Science. 1993 Mar 12;259(5101):1622-5. PMID: https://www.ncbi.nlm.nih.gov/pubmed/8456286
- ↑ Atlas of genetics & cytogenetics in oncology & haematology http://atlasgeneticsoncology.org/genes/SPI1ID269.html