non-POU domain-containing octamer-binding protein; nonO protein; 54 kD nuclear RNA- & DNA-binding protein; 55 kD nuclear protein; DNA-binding p52/p100 complex, 52 kD subunit; NMT55; p54(nrb); p54nrb (NONO, NRB54)

Function

• DNA- & RNA binding protein
• involved in several nuclear processes
• binds the conventional octamer sequence in double stranded DNA
• also binds single-stranded DNA & RNA at a site independent of the duplex site (putative)
• involved in pre-mRNA splicing, probably as an heterodimer with SFPQ
• interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b
• the SFPQ-NONO
• heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs
• the SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1
• the SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair & V(D)J recombination & may stabilize paired DNA ends
• in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates & cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex
• nono is involved in transcriptional regulation
• the SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter & regulates basal & cAMP-dependent transcriptional activity
• NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) & activates transcription (putative)
• monomer & component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kD (NONO) & 2 100 kDa (SFPQ) subunits
• NONO is a component of spliceosome & U5.4/6 snRNP complexes interacts with PSPC1 & SNRPA/U1A
• part of complex consisting of SFPQ, NONO & MATR3
• part of a complex consisting of SFPQ, NONO & NR5A1
• part of a complex consisting of SFPQ, NONO & TOP1
• interacts with SPI1 (putative)
• interacts with RNF43

Structure

• contains 2 RRM domain (RNA recognition motif)s

Compartment

nucleus

Expression

• expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney & pancreas
• also found in a number of breast tumor cell lines

Pathology

• chromosomal translocation t(X;X)(p11.2;q13.1) involving NONO with TFE3 may be a cause of papillary renal cell carcinoma (PRCC)

More general terms

• DNA-binding protein
• RNA-binding protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=4841
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:4841
• OMIM: https://mirror.omim.org/entry/300084
• UniProt: http://www.uniprot.org/uniprot/Q15233.html