transcription factor spi-B (SPIB)

Function

• sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer
• promotes development of plasmacytoid dendritic cells
• may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development & antigenic stimulation
• can form homotypic interactions
• interacts with IRF4
• may also interact with CREBBP, EP300, SPI1/PU.1 related, JUN & TBP

Structure

• the protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in Pro, Ser & Thr
• each of these domains may be required for transcriptional activation of a subset of target genes
• belongs to the ETS family
• contains 1 ETS DNA-binding domain

Compartment

• isoform 1: nucleus
• isoform 2: cytoplasm

Alternative splicing

named isoforms=2

Expression

• expressed in plasmacytoid dendritic cells & B-cells
• not expressed in T-cells or granulocytes
• may also be enriched in stem cell populations of the liver

More general terms

• Ets family protein

Database

• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:6689
• OMIM: https://mirror.omim.org/entry/606802
• UniProt: http://www.uniprot.org/uniprot/Q01892.html