CLIP-associating protein 1; cytoplasmic linker-associated protein 1; multiple asters homolog 1; protein Orbit homolog 1; hOrbit1 (CLASP1, KIAA0622, MAST1)

^[1]

Function

regulation of microtubule dynamics (density, length distribution & stability of interphase microtubles
participates in generating polarized microtubule networks
component of kinetochore outer corona region
mediates interactions between microtubule plus ends & cell cortex
microtubule plus-end tracking protein
promotes stabilization of dynamic microtubules
role in nucleation of noncentrosomal microtubules originating from the trans-Golgi network
required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell
may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 & PHLDB2
this cortical microtubule stabilizing activity is regulated, at least in part, by phosphatidylinositol 3-kinase signaling
also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle
phosphorylated upon DNA damage, probably by ATM or ATR
interacts with CLIP2, ERC1, MAPRE1, MAPRE3, microtubules, PHLDB2 & RSN
interaction with ERC1 may be mediated by PHLDB2
interacts with GCC2;
recruits CLASP1 to Golgi membranes
interacts with MACF1 (putative)

cytoplasm, cytoskeleton, centrosome
chromosome, centromere, kinetochore, spindle
Golgi apparatus, trans-Golgi network
localizes preferentially near plus ends of growing spindle microtubules
localizes to centrosomes, kinetochores & the mitotic spindle from prometaphase
subsequently localizes to the spindle midzone from anaphase & to the midbody from telophase
in migrating cells, localizes to the plus ends of microtubules within the cell body & to the entire microtubule lattice within the lamella
localizes to the cell cortex & this requires ERC1 & PHLDB2