centromere protein F; CENP-F; AH antigen; kinetochore protein CENPF; mitosin (CENPF)

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Function

required for kinetochore function & chromosome segregation in mitosis
required for kinetochore localization of dynein, LIS1, NDE1 & NDEL1
regulates recycling of the plasma membrane by acting as a link between recycling vesicles & the microtubule network though its association with STX4 & SNAP25
acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating activity of RB protein during cell division & proliferation
may play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle & in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes
interaction with RB directs embryonic stem cells toward a cardiac lineage
involved in the regulation of DNA synthesis & hence cell cycle progression, via its C-terminus
potential role regulating skeletal myogenesis & in cell differentiation in embryogenesis
role in dendritic cell regulation of T-cell immunity against Chlamydia
hyperphosphorylated during mitosis
phosphorylated upon DNA damage, probably by ATM or ATR
interacts with & STX4 (via C-terminus) (putative)
interacts (via N-terminus) with RBL1, RBL2 & SNAP25
self-associates
interacts with CENP-E & BUBR1 (via C-terminus)
interacts (via C-terminus) with NDE1, NDEL1 & RB1

cytoplasm, cytoskeleton, spindle
perinuclear region, nuclear matrix
chromosome, centromere, kinetochore
relocalizes to the kinetochore/centromere (coronal surface of the outer plate) & the spindle during mitosis
observed in nucleus during interphase
not in the nucleolus during interphase
at metaphase becomes localized to the kinetochore & mitotic apparatus as well as cytoplasm
by telophase, is concentrated within the intracellular bridge at either side of the mid-body

gradually accumulates during the cell cycle, reaching peak levels in G2 & M phase, & is rapidly degraded upon completion of mitosis