Ras association domain-containing protein 1 (RASSF1, RDA32)

Function

• tumor suppressor (putative)
• death receptor-dependent apoptosis
• when associated with MOAP1, promotes BAX translocation to mitochondrial membranes in response to TNF & TNFSF10
• inhibits proliferation
• negatively regulates cell cycle progression G1/S-phase transition - regulates cyclin D1 protein
• inhibits activation of STK3 & STK4
• scaffold protein (putative)
• interacts with XPAl, CDC20 (during prometaphase), STK3, STK4, NFRSF1A, TNFRSF10A, RSSF5, HRAS1, RSSF1, XPA

Compartment

• isoform A localizes to microtubules during interphase, centrosomes associated with microtubules during prophase, spindle fibers & spindle poles at metaphase & anaphase, midzone during early telophase, midbody in late telophase in cells
• isoform C is predominantly nuclear

Alternative splicing

7 alternate splice forms, A-G

Expression

• isoform A & isoform C are ubiquitously expressed
• isoform B is expressed in hematopoietic cells

Pathology

• loss of RASSF1A seen in small cell lung cancer & some other cancer cell lines which correlate with hypermethylation of the CpG island promoter sequence of RASSF1A

More general terms

• zinc finger protein
• Ras association domain-containing protein (RASSF)

Database

• OMIM: https://mirror.omim.org/entry/605082
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=11186
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:11186
• UniProt: http://www.uniprot.org/uniprot/Q9NS23.html