E3 ubiquitin-protein ligase RNF216; RING finger protein 216; ubiquitin-conjugating enzyme 7-interacting protein 1; zinc finger protein inhibiting NF-kappa-B; triad domain-containing protein 3 (RNF216, TRIAD3, UBCE7IP1, ZIN)

Function

• isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, & then transfers it to substrates promoting their degradation by the proteasome
• promotes degradation of TLR4 & TLR9
• isoform 3/ZIN inhibits TNF & IL-1 mediated activation of NF-kappa-B
• promotes TNF & RIP mediated apoptosis
• protein modification; protein ubiquitination
• auto-ubiquitinated
• interacts with UBE2L3 & to some extent with UBE2L6
• interacts with TLR3, TLR4, TLR5 & TLR9
• isoform 3/ZIN binds RIPK1 & HIV VIF

Structure

• the RING-type Zn+2 finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (putative)
• contains 1 IBR-type Zn+2 finger
• contains 2 RING-type Zn+2 fingers

Compartment

cytoplasm

Alternative splicing

• named isoforms=3
• 4 different alternatively spliced mRNAs

Expression

• ubiquitous
• highest levels of expression in testis & peripheral blood leukocytes

More general terms

• ubiquitin conjugating enzyme 7 interacting protein
• E3 ubiquitin ligase
• ring finger protein
• phosphoprotein

Additional terms

• ubiquitin (UBCEP2, UBB, UBC)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=54476
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:54476
• OMIM: https://mirror.omim.org/entry/609948
• UniProt: http://www.uniprot.org/uniprot/Q9NWF9.html