Ena/VASP-like protein (Ena/vasodilator-stimulated phosphoprotein- like) (EVL RNB6)

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Function

enhances actin nucleation & polymerization
required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes
binds to the SH3 domains of ABL1, LYN & SRC
binds to profilin, with preference for isoform IIa of PFN2, & the WW domain of APBB1/FE65
binds to SEMA6A
interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP
interacts with RAPH1
binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA
phosphorylated by PKA, phosphorylation abolishes binding to SH3 domains of ABL & SRC

homotetramer
belongs to the Ena/VASP family
contains 1 WH1 domain
EVH2 domain is comprised of 3 regions
- block A is a thymosin-like domain required for G-actin binding, the KLKR motif within this block is essential for the G-actin binding & for actin polymerization
- block B is required for F-actin binding & subcellular location
- block C is required for tetramerization

cytoplasm, cytoskeleton
cell projection, lamellipodium
targeted to the leading edge of lamellipodia & the dital tip of stress fibers
in activated T-cells, localizes to the F-actin collar & the distal tip of microspikes (putative)