Ras-associated & pleckstrin homology domains-containing protein 1; RAPH1; ALS 2 chromosomal region candidate gene 9/18 protein; lamellipodin; proline-rich EVH1 ligand 2; PREL-2; protein RMO1 (RAPH1, ALS2CR18, ALS2CR9, KIAA1681, LPD, PREL2, RMO1)

Function

• mediator of localized membrane signals
• regulation of lamellipodial dynamics
• negatively regulates cell adhesion
• interacts with EVL & VASP and targets them to the leading edge

Structure

• belongs to the MRL family
• contains 1 PH domain
• contains 1 Ras-associating domain

Compartment

• cell membrane, peripheral membrane.
• recruited to the membrane, via the PH domain, by PI(3,4)P2
• colocalizes with VASP at the tips of lamellipodia & filopodia
• also colocalizes with pathogens, Vaccinia & enteropathogenic E.col at the interface between the pathogen & their actin

Alternative splicing

named isoforms=9

Expression

• isoform RMO1-RAPH1 is ubiquitously expressed; highest levels in brain, heart, ovary, developing embryo
• isoform RMO1 is widely expressed; highest levels in liver
• low expression in B-cells

Pathology

down-regulated in some cancers

• diminished expression in metastatic osteosarcomas relative to primary osteosarcoma tumors
• down-regulated in 43% of breast cancers
• down-regulated in 25% of ovarian cancers

More general terms

• cytoskeletal protein
• phosphoprotein

Additional terms

• amyotrophic lateral sclerosis (ALS); Lou Gerig's disease
• amyotrophic lateral sclerosis [ALS] 2 chromosomal region candidate gene

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=65059
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:65059
• OMIM: https://mirror.omim.org/entry/609035
• UniProt: http://www.uniprot.org/uniprot/Q70E73.html