transcription factor E2F2 (E2F-2, E2F2)

Function

• transcription activator
• binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication
• the DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase
• E2F2 binds specifically to RB1 protein (cell-cycle dependent)
• component of the DRTF1/E2F transcription factor complex
• forms heterodimers with DP family members
• E2F2 complexes bind specifically hypophosphorylated retinoblastoma protein RB1
• during the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active
• binds EAPP
• phosphorylated by CDK2 & cyclin A-CDK2 in the S-phase

Structure

• DNA-binding-domain in the central part of the molecule
• C-terminal Rb-binding domain
• belongs to the E2F/DP family

Compartment

nucleus

Expression

• expressed in placenta > lung
• expressed in manyimmortalized cell lines derived from tumors

Pathology

• viral oncoproteins, notably E1A, T-antigen & HPV E7, are capable of sequestering RB protein, thus releasing active E2F2 complexes

More general terms

• E2F

Database

• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:1870
• OMIM: https://mirror.omim.org/entry/600426
• UniProt: http://www.uniprot.org/uniprot/Q14209.html
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=1870