serine/threonine protein kinase WNK2; antigen NY-CO-43; protein kinase lysine-deficient 2; protein kinase with no lysine 2; serologically defined colon cancer antigen 43 (WNK2, KIAA1760, PRKWNK2, SDCCAG43, P/OKcl13)
Jump to navigation
Jump to search
Function
- serine/threonine protein kinase
- role in regulation of electrolyte homeostasis, cell signaling, survival, & proliferation
- activator of Na+-coupled chloride cotransporters
- inhibitor of K+-coupled chloride cotransporters
- activates SLC12A2, SCNN1A, SCNN1B, SCNN1D & SGK1
- inhibits SLC12A5
- negatively regulates EGF-induced activation of the ERK/MAPK-pathway & downstream cell cycle progression
- affects MAPK3/MAPK1 activity by modulating activity of MAP2K1
- modulation depends on phosphorylation of MAP2K1 by PAK1
- WNK2 acts by interfering with activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA & RAC1, which display reciprocal activity
- activation requires autophosphorylation of Ser-356
- phosphorylation of Ser-352 also promotes increased activity
- autophosphorylated
- forms a complex with the phosphorylated form of STK39
Structure
- belongs to the protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily
- contains 1 protein kinase domain
Compartment
Alternative splicing
named isoforms=4
Expression
- expressed in various cancer cell lines (at protein level)
- predominantly expressed in heart, brain, skeletal muscle & colon
Notes
- Cys-224 is present instead of the conserved Lys within the kinase domain
- Lys-207 appears to fulfill the required catalytic function