nuclear body (nuclear domain 10, PML oncogenic domain, POD, nuclear dot, KR body)

Function

• seeems to be involved in transcriptional regulation
• DAXX seems to regulate transcription in PML nuclear bodies together with transcription factor PML & may influence TNFRSF6-dependent apoptosis
• role in checkpoint responses upon DNA damage
• heterochromatin remodelling in G2 phase

Structure

• diameters between 0.2 & 1 mm
• number & size change during the cell cycle
• electron microscopic analysis show that the nuclear body is shaped like a doughnut
• components of the nuclear body include:
  • transcription factor PML
    • sumoylation is necessary for PML nuclear body formation
  • nuclear body protein SP140
  • RB protein
  • protein p53
  • CBP
  • DNA helicase BLM22
  • eIF-4
  • INT-6
  • Daxx
  • SUMO-1
  • HAUSP
  • Sp100

Expression

• cells typically contain 10-30 nuclear bodies per nucleus

Notes

• discrete nuclear speckles containing transcription factor PML along with other proteins, which were originally identified as autoantigens in primary biliary cirrhosis patients

More general terms

• intracellular compartment (intracellular space)

Component of

• cell nucleus