Rac GTPase-activating protein 1; male germ cell Racgap; MgcRacGAP; protein CYK4 homolg; CYK4; HsCYK-4 (RACGAP1, KIAA1478, MGCRACGAP)

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Function

component of the centralspindlin complex
embryogenesis, early stages
microtubule-dependent steps in cytokinesis (putative)
cell growth & differentiation of hematopoietic cells (not via regulating Rac GTPase activity)
growth-related processes in adipocytes, myoblasts
spermatogenesis
role in neuronal proliferation via RACGAP1 pathway
GAP activity towards CDC42, RAC1 > RHOA
associates with alpha-tubulin, beta-tubulin & gamma-tubulin & microtubules
may play a role in regulating cortical activity through RHOA during cytokinesis
may participate in the regulation of sulfate transport in male germ cells
phosphorylated at multiple sites in the midbody during cytokinesis
associates with AURKB during M phase
phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA
phosphorylation on multiple Ser by PLK1 enhances its association with ECT2 & is critical for cleavage furrow formation
interacts via its Rho-GAP domain with RND2
interacts via its Rho-GAP domain & basic region with PRC1
interaction with PRC1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology
interacts with SLC26A8 via its N-terminus
interacts with RAB11FIP3
interacts with ECT2
- occurs at anaphase & during cytokinesis (microtubule-dependent)
- enhanced by phosphorylation by PLK1

the coiled coil region is indispensible for localization to the midbody during cytokinesis
contains 1 phorbol-ester/DAG-type Zn+2 finger
contains 1 Rho-GAP domain

expressed in testis, thymus, placenta > spleen, peripheral blood lymphocytes
in testis, expression is restricted to germ cells; highest levels in spermatocytes
expression regulated in a cell cycle-dependent manner; peaks during G2/M phase