Rab11 family-interacting protein 3; Rab11-FIP3; FIP3-Rab11; EF hands-containing Rab-interacting protein; eferin; arfophilin-1; MU-MB-17.148 (RAB11FIP3, ARFO1, KIAA0665)

Function

• acts as a regulator of endocytic traffic by participating in membrane delivery
• required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis
• also required for the structural integrity of the endosomal recycling compartment during interphase
• may play a role in breast cancer cell motility by regulating actin cytoskeleton
• forms a complex with Rab11 (RAB11A or RAB11B) & ARF6
• interacts with RAB11A; the interaction is direct
• interacts with RAB11B, RAB25 & RAB11FIP4
• interacts with ARF6
• specifically interacts with ARF6
• interacts with RACGAP1/MgcRacGAP; interaction takes place during late stage of cytokinesis & is required for recruitment to the midbody
• interacts with ASAP1 & EXOC7

Structure

• homodimer
• the RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B)
• contains 2 EF-hand domains
• contains 1 RBD-FIP domain

Compartment

• recycling endosome membrane; peripheral membrane protein
• cytoplasm, cytoskeleton, centrosome
• in early mitosis, remains diffuse & distributed throughout cell
• the onset of anaphase sequesters endosomes (veiscles) to the centrosomes at the opposite poles of the cell.
• during telophase these vesicles move from the centrosomes, to the furrow, & then to the midbody to aid in abscission
• interaction with Rab11 mediates localization to endosomes
• interaction with ARF6 mediates localization to the midbody

Alternative splicing

named isoforms=3

More general terms

• Ca+2 binding protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9727
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:9727
• OMIM: https://mirror.omim.org/entry/608738
• UniProt: http://www.uniprot.org/uniprot/O75154.html