endothelial differentiation-related factor 1; EDF-1; multiprotein-bridging factor 1 (MBF1, EDF1)

Function

• transcriptional coactivator
• stimulates NR5A1 & ligand-dependent NR1H3/LXRA & PPARG transcriptional activities
• enhances the DNA-binding activity of ATF1, ATF2, CREB1 & NR5A1
• regulates nitric oxide synthase activity via sequestering calmodulin in the cytoplasm
  • binding to calmodulin is regulated by Ca+2 & phosphorylation of the IQ motif
• endothelial cell differentiation
• hormone-induced cardiomyocyte hypertrophy
• lipid metabolism
• interacts with TBP & the transcription factor IID complex
  • interaction with TBP is regulated by phosphorylation
• interaacts with NR5A2, NR1H3 & PPARG.
• binds NR5A1, ATF1, FOS & JUN via their conserved basic region
• phosphorylated by PKA & PKC

Compartment

cytoplasm, nucleus

Alternative splicing

named isoforms=2

Expression

• ubiquitously expressed
• expressed in brain, liver, lung, kidney, heart, pancreas, intestine, adipose tissues
• expressed in fetal tissues

Pathology

• down-regulated by HIV type 1 protein TAT or phorbol ester treatment in endothelial cells

More general terms

• phosphoprotein
• nuclear protein

Database

• OMIM: https://mirror.omim.org/entry/605107
• UniProt: http://www.uniprot.org/uniprot/O60869.html