epsin-1 (EPS-15-interacting protein 1, EH domain-binding mitotic phosphoprotein, EPN1)

Function

• binds to membranes enriched in phosphatidylinositol-4,5- biphosphate (PIP2)
• modifies membrane curvature & facilitates formation of clathrin-coated invaginations
• regulates receptor-mediated endocytosis
• binds clathrin, ZBTB16/ZNF145 & ITSN1
• binds ubiquitinated proteins
• binds REPS2, EPS15, AP2A1, AP2A2
• NPF repeat domain is involved in EPS15 binding
• DPW repeat domain is involved in AP2A2 & clathrin binding
• phosphorylated on Ser &/or Thr in mitotic cells
• phosphorylation diminishes interaction with REPS2, AP-2 & membrane
• depolarization of synaptosomes results in dephosphorylation
• ubiquitinated

Structure

• belongs to the epsin family
• contains 1 ENTH domain (epsin N-terminal homology domain)
  • contains 2 UIM repeats (ubiquitin-interacting motif)

Compartment

• cytoplasm, peripheral membrane, nucleus, clathrin-coated pit
• colocalizes with clathrin & AP-2 on the plasma membrane
• detected in presynaptic nerve terminals & in Golgi stacks
• may shuttle to the nucleus when associated with ZBTB16/ZNF145

Alternative splicing

named isoforms=3

More general terms

• other protein

Database

• OMIM: https://mirror.omim.org/entry/607262
• UniProt: http://www.uniprot.org/uniprot/Q9Y6I3.html