NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; ubiquitin-activating enzyme E1C; ubiquitin-like modifier-activating enzyme 3; ubiquitin-activating enzyme 3 (UBA3, UBE1C)

Function

• NEDD8-specific E1 complex
• catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme
• E1 activates NEDD8 by first adenylating its C-terminal Gly with ATP, thus linking NEDD8-Gly to UBA3's catalytic Cys, yielding a NEDD8-UBA3 thioester & AMP
• E1 finally transfers NEDD8 to the catalytic Cys of UBE2M
• down-regulates steroid receptor activity
• necessary for cell cycle progression
• binding of TP53BP2 to the regulatory subunit
• NAE1 decreases activity
• protein modification; protein neddylation
• heterodimer of UBA3 & NAE1
• interacts with NEDD8, UBE2F & UBE2M.
• binds ESR1 & ESR2 with bound steroid ligand

Structure

• belongs to the ubiquitin-activating E1 family, UBA3 subfamily

Alternative splicing

named isoforms=2

Expression

• ubiquitously expressed

Notes

• Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin

More general terms

• ligase or synthetase
• oligomerizing protein

Additional terms

• NEDD8; ubiquitin-like protein Nedd8; neddylin; neural cell expressed developmentally down-regulated protein 8; NEDD-8 (NEDD8)
• ubiquitin (UBCEP2, UBB, UBC)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9039
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:9039
• OMIM: https://mirror.omim.org/entry/603172
• UniProt: http://www.uniprot.org/uniprot/Q8TBC4.html