carbohydrate sulfotransferase 2; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1; GlcNAc6ST-1; Gn6ST-1 (CHST2, GN6ST)

Function

• catalyzes transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans & within mucin-associated glycans that can ultimately serve as L-selectin ligands
• L-selectin ligands are present in high endothelial cells & play a role in lymphocyte homing at sites of inflammation
• role in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X & in lymphocyte homing to Peyer patches
• no activity toward O-linked sugars
• substrate specificity may be influenced by its subcellular location
• sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains

Kinetic parameters:

• KM=3.9 uM for PAPS
• KM=1.4 mM for betaBnO-GlcNAc

Structure

• homodimer; disulfide-linked
  • homodimerization is not essential for enzyme activity
• belongs to the sulfotransferase 1 family gal/GlcNAc/galNAc subfamily

Compartment

• Golgi, trans-Golgi network membrane
• single-pass type 2 membrane protein (probable)

Alternative initiation

Named isoforms=2

Expression

• widely expressed
• highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary & placenta
• expressed by high endothelial cells & leukocytes
• up-regulated upon cytokine activation

Notes

• uncertain whether Met-1 or Met-48 is the initiator

More general terms

• carbohydrate sulfotransferase (CHST)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9435
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:9435
• OMIM: https://mirror.omim.org/entry/603798
• UniProt: http://www.uniprot.org/uniprot/Q9Y4C5.html